synthetic biology
Posts tagged “synthetic biology”.
Comparative genomics of flavivirus 3' UTR RNA structures
A comparative genomics analysis of flavivirus 3' UTRs that identifies conserved exoribonuclease-resistant RNAs and lineage-specific architectural variation across tick-borne, insect-specific, and no-known-vector flaviviruses.
Co-transcriptional riboswitch modeling with ViennaRNA
A landscape-based method for modeling how cotranscriptional folding and ligand binding interact in kinetically controlled riboswitches, illustrated with the 2'dG riboswitch from Mesoplasma florum.
In silico design of ligand-triggered RNA switches
A computational workflow for designing ligand-triggered RNA switches, with emphasis on sequence design, folding kinetics, and candidate prioritization.
Co-transcriptional folding and metastable states in riboswitch function
This paper uses NMR spectroscopy to resolve transcription intermediates of the 2'dG riboswitch at single-nucleotide resolution, showing how transcript length and metastable states govern ligand-controlled switching.